Contact an Account ManagerThe bacterial phosphoenolpyruvate-dependent phosphotransferase system. Isolation of active site peptides by reversed phase HPLC and determination of their primary structure
Summary
Type: HPLC
Application #: 108190
Catalog#: 720022.46 - MN HPLC Column, Nucleosil C8, 100A, 10um, 4.6x250mm
Detection: A) UV, 230nm, B) radioactivity (32P)
Conditions: eluent A: 20mM K phosphate pH 6.8, eluent B: 85% acetonitrile, linear gradient from 0 to 60% B in 60min
flow rate 1ml/min
Authors: Alpert CA
Source: J Chromatogr 326 (1985) 363 - 371
Details
Legend: Fig. 3. A) Gradient elution of Lys-C proteolytic fragments of 32P-labelled
phosphoenzyme I (Streptococcus lactis), asterisk indicates position of 32P
label in the UV track, B) distribution of the 32